Fluorescence in situ hybridization: Blood, Skin and Other Tissues
RNA-ISH & Confocal Laser Microscopy
(c) 2022 T Lab Inc
These Research Use Only (RUO) tests combine advanced RNA-fluorescence in situ hybridization (FISH) technology and confocal laser microscopy to visualize target RNA sequences in blood, skin, and other tissues, and develop tests to evaluate the mechanisms of inflammation and disease at the cellular level.
These tests are currently provided for research use only, not intended for diagnosis or clinical decision making.
RNA-ISH tests are for "Research Use Only"
(c) 2022 T Lab Inc
What are "Molecular Imaging" tests?
We use a technology known as "FISH" (which is an acronym for Fluorescence in Situ Hybridization) to detect the presence of a pathogen's genetic (DNA or RNA) specific signature in blood and skin specimens. In FISH testing, highly specific molecules called probes bind to the sequence of interest and are used to attach dyes that fluoresce when struck by laser light at a specific frequency. Images can then be produced by a confocal laser microscope.
Why RNA probes?
RNA is thought to have a shorter half-life in blood, therefore, a positive RNA FISH result is more likely to reflect an active infection.
Why robosomal RNA (rRNA) probes?
rRNA is an important constituent of ribosomes, which are the "factory" where proteins are synthesized by living organisms. rRNA is among the most abundant RNA in cells, allowing probes for rRNA the ability to more efficiently detect microorganisms.
What is confocal scanning laser microscopy?
Confocal scanning laser microscopy is a technique that uses a laser to scan multiple layers in a sample of blood or tissue, which can then be collapsed or merged to make a 3D image of the sample. It allows for quantifiable imaging of samples that are thicker than could be used in a conventional microscope.